The Trakai Kenassa serves the Karaite community, a small Turkic-speaking religious and ethnic group which presently number about 50 people in the town. Lithuanian Karaite religion is based on the Jewish faith but has some distinct differences. Amongst these, and related to the Kenassa which had many similarities to a synagogue and has a similar layout, was the requirement to remove footwear before entering the building.
There were once 20 of these meeting houses and this one represents a rare extant and, the only wooden example of a kenassa possessing an interior dome. The only other extant example is located in Vilnius.